研究概述：

    表观遗传与生物信息学实验室

    实验室主要研究领域包括：（1）肿瘤表观遗传重编程机制，（2）基于液体活检cfDNA甲基化的肿瘤早期无创诊断技术，（3）神经性疾病的表观遗传重编程机制。

    利用分子生物学、细胞生物学、高通量测序、生物信息学、动物模型等技术，探索疾病发生的表观遗传学机制、鉴定用于疾病诊断和预后的分子标志物和新的治疗靶点。

发表论文：

1. Li HT^*, Jang HJ^*, Rohena-Rivera K^*, Liu M, Gujar H, Kulchycki J, Zhao S, Billet S, Zhou X, Weisenberger DJ, Gill I, Jones PA, Bhowmick NA, Liang G. RNA mis-splicing drives viral mimicry response after DNMTi therapy in SETD2-mutant kidney cancer. Cell Rep. 2023 Jan 31;42(1):112016.

2. Li HT^*, Xu L^*, Weisenberger DJ, Li M, Zhou W, Peng CC, Stachelek K, Cobrinik D, Liang G, Berry JL. Characterizing DNA methylation signatures of retinoblastoma using aqueous humor liquid biopsy. Nat Commun. 2022 Sep 21;13(1):5523.

3. Zhou X, Sekino Y, Li HT, Fu G, Yang Z, Zhao S, Gujar H , et al. Dual Inhibition of DNA methylation and PARP Increases Anti-tumor Efficacy in SETD2-Deficient Kidney Cancer. Cancer Research. 2023 Sep 11. Online ahead of print.

4. Zhang L, Li HT, Shereda R, Lu Q, Weisenberger DJ, O'Connell C, Machida K, An W, Lenz HJ, El-Khoueiry A, Jones PA, Liu M, Liang G. DNMT and EZH2 inhibitors synergize to activate therapeutic targets in hepatocellular carcinoma. Cancer Lett. 2022 Nov 1;548:215899.

5. Gujar H, Mehta A, Li HT^*, Tsai YC, Qiu X, Weisenberger DJ, Jasiulionis MG, In GK, Liang G. Characterizing DNA methylation signatures and their potential functional roles in Merkel cell carcinoma. Genome Med. 2021 Aug 16;13(1):130.

6. Xu T, Li HT^*, Wei J, Li M, Hsieh TC, Lu YT, Lakshminarasimhan R, Xu R, Hodara E, Morrison G, Gujar H, Rhie SK, Siegmund K, Liang G, Goldkorn A. Epigenetic plasticity potentiates a rapid cyclical shift to and from an aggressive cancer phenotype. Int J Cancer. 2020 Jun 1;146(11):3065-3076.

7. Liu M, Zhang L, Li H^*, Hinoue T, Zhou W, Ohtani H, El-Khoueiry A, Daniels J, O'Connell C, Dorff TB, Lu Q, Weisenberger DJ, Liang G. Integrative Epigenetic Analysis Reveals Therapeutic Targets to the DNA Methyltransferase Inhibitor Guadecitabine (SGI-110) in Hepatocellular Carcinoma. Hepatology. 2018 Oct;68(4):1412-1428.

8. Li HT, Duymich CE, Weisenberger DJ, Liang G. Genetic and Epigenetic Alterations in Bladder Cancer. Int Neurourol J. 2016 Nov;20(Suppl 2):S84-94.

9. Li HT, Gong T, Zhou Z, Liu YT, Cao X, He Y, Chen CD, Zhou JQ. Yeast Hmt1 catalyses asymmetric dimethylation of histone H3 arginine 2 in vitro. Biochem J. 2015 May 1;467(3):507-15..

主持课题：

山东省自然科学基金面上项目，基于尿液游离DNA甲基化的泌尿系统肿瘤的无创诊断技术，2025-01至2027-12，10万；